Toxoplasma IgG ELISA Kit

Inkcazelo emfutshane:

I-Toxoplasma IgG ELISA Kit yi-enzyme-edityaniswe ne-immunosorbent assay yokufumanisa umgangatho we-IgG-class antibodies kwi-Toxoplasma kwi-serum yabantu okanye i-plasma.Yenzelwe ukuba isetyenziswe kwiilabhoratri zeklinikhi zokuxilongwa kunye nokulawulwa kwezigulane ezinxulumene nosulelo lwe-Toxoplasma.Ngokubanzi, ukunyuka kwe-antibodies ye-Toxoplasma IgM kubonakala kuqala emva kosulelo lwe-Toxoplasma, enokuthi ibonwe malunga neentsuku ezi-6 emva kosulelo, kwaye ngokuthe ngcembe iyenyuka kwaye ihlale iiveki eziliqela ukuya kwiinyanga, kwaye ekugqibeleni iyancipha de iphele.I-antibody ye-IgG echanekileyo ibonisa usulelo lwangaphambili lwe-Toxoplasma gondii, oludla ngokungapheliyo, kodwa lunokuba lubukhali.Emva kovavanyo lokuqala lwe-antibodies ye-IgG, uvavanyo olufanayo luphinda luphindwe emva kweeveki ezi-2-3 kwaye ukuba kukho ukunyuka kwe-4 okanye ngaphezulu kokunyuka kwamandla e-antibodies, ukuxilongwa okuqhubekayo kuyadingeka.

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Iinkcukacha zeMveliso

Iithegi zeMveliso

Umgaqo

Le khithi ibona i-antibody ye-Toxoplasma lgG (TOX-lgG) kwi-serum yabantu okanye iisampulu zeplasma, imicu ye-polystyrene microwell ifakwe ngaphambili kunye ne-Toxoplasma antigen.Emva kokuqala ukongeza i-serum okanye iisampuli ze-plasma eziza kuhlolwa, ii-antibodies ezihambelanayo ezihambelanayo (i-TOX-lgG-Ab & ezinye i-lgM-Ab) ezikhoyo kwiimpawu zesigulane zibophelela kwii-antigens kwisigaba esiqinileyo, kunye nezinye izinto ezingabotshwanga ziya kususwa ngokuhlamba.Kwinqanaba lesibini, i-HRP (i-horseradish peroxidase) -i-conjugated anti-human lgG iya kusabela ngokukodwa kuphela nge-TOX lgG antibodies.Emva kokuhlamba ukususa i-HRP-conjugate engabotshwanga, izisombululo ze-chromogen zongezwa kumaqula.Phambi kwe (TOX Ag) - (TOX-lgG) - (anti-human lgG-HRP) immunocomplex, emva kokuhlamba ipleyiti, i-substrate TMB yongezwa kuphuhliso lombala, kunye ne-HRP eqhagamshelwe kwi-complex ibangela ukusabela komphuhlisi wombala. ukuvelisa into eluhlaza, yongeza i-50 µ I yeStop Solution, kwaye ujike ube tyheli.Ubukho be-absorbection ye-antibody ye-TOX-lgG kwisampulu yagqitywa ngumfundi we-microplate.

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Ukucaciswa kweMveliso

Umgaqo	Uvavanyo lwe-Enzyme olunxibelelene ne-immunosorbent
Uhlobo	Indlela engathanga ngqo
Isatifikethi	NMPA
Umzekelo	Iserum yabantu / iplasma
Inkcazo	48T / 96T
Ubushushu bokugcina	2-8℃
Beka ubomi kwishelufa	Iinyanga ezili-12