Herpes Simplex II IgM ELISA Kit

Inkcazelo emfutshane:

I-Herpes Simplex II IgM ELISA Kit yi-enzyme-edityaniswe ne-immunosorbent assay yokufumanisa umgangatho we-IgM-class antibodies kwi-Herpes Simplex virus II kwi-serum yabantu okanye i-plasma.I-Herpes Simplex Virus (HSV) iyinxalenye ye-A virus subfamily yosapho lwe-Herpesviridae kwaye inobukhulu be-particle malunga ne-180 nm.Intsholongwane okwangoku ihlelwa njengohlobo lwe-I kunye nohlobo lwe-II ngokusekelwe kumahluko kwi-antigenicity.Iintsholongwane ze-Herpes simplex zixhaphake phakathi kwabemi kwaye abantu kuphela abamkeli.Uhlobo lwe-HSV II lusasazwa ikakhulu ngokwabelana ngesondo.Inxulumene ikakhulu nosulelo lwangaphandle kunye nosulelo lwe-neonatal kwaye lunokubangela i-herpes yangasese kunye ne-herpes ye-neonatal.Ukuba umfazi okhulelweyo unosulelo oluphambili lwentsholongwane ye-herpes, intsholongwane inokusulela umntwana kwi-placenta kwaye yenze usulelo lokuzala.Ukuba usulelo oluyiprayimari okanye intsholongwane ye-herpes ephindaphindiweyo ikhona kumjelo wokuzalwa komama, inokuchaphazela umntwana osandul 'ukuzalwa ngexesha lokubeletha kwaye kubangele usulelo lwe-neonatal.


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Umgaqo

Le khithi ibona i-Herpes Simplex virus II IgM antibody (HSV2-IgM) kwi-serum yabantu okanye iisampulu zeplasma, imicu ye-polystyrene microwell ifakwe ngaphambili kunye nezilwa-buhlungu ezijoliswe kwiprotheni yomntu ye-immunoglobulin M (anti-µ chain).Emva kokuqala ukongeza i-serum okanye iisampuli ze-plasma eziza kuhlolwa, ii-antibodies ze-IgM kwi-specimen zingabanjwa, kunye nezinye izinto ezingabotshwanga (kubandakanywa ne-IgG antibodies) ziya kususwa ngokuhlamba.Kwinqanaba lesibini, i-HRP (i-horseradish peroxidase) -i-antigens edibeneyo iya kusabela ngokukodwa kuphela nge-HSV2 IgM antibodies.Emva kokuhlamba ukususa i-HRP-conjugate engabotshwanga, izisombululo ze-chromogen zongezwa kumaqula.Phambi kwe (anti-µ)-(HSV2-lgM)-(HSV2 Ag-HRP) immunocomplex, emva kokuhlamba ipleyiti, i-substrate ye-TMB yongezwa kuphuhliso lombala, kwaye i-HRP edityaniswe kwi-complex ibangela ukusabela komphuhlisi wombala velisa into eluhlaza, yongeza i-50 µ I yeStop Solution, kwaye ujike ube tyheli.Ubukho bokuthatha i-antibody ye-HSV2-IgM kwisampulu yagqitywa ngumfundi we-microplate.

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Uvakalelo oluphezulu, ukuchaneka kunye nokuzinza

Ukucaciswa kweMveliso

Umgaqo Uvavanyo lwe-Enzyme olunxibelelene ne-immunosorbent
Uhlobo Indlela yokubamba
Isatifikethi NMPA
Umzekelo Iserum yabantu / iplasma
Inkcazo 48T / 96T
Ubushushu bokugcina 2-8℃
Beka ubomi kwishelufa Iinyanga ezili-12

Ukuodola Ulwazi

Igama lemveliso Pakisha Umzekelo
Herpes Simplex II IgM ELISA Kit 48T / 96T Iserum yabantu / iplasma

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