Human Cytomegalovirus IgM ELISA Kit

Inkcazelo emfutshane:

I-Human Cytomegalovirus IgM (HCMV-IgM) I-ELISA Kit yi-enzyme-linked immunosorbent assay yokufumanisa umgangatho we-IgM-class antibodies kwi-Human Cytomegalovirus kwi-serum yabantu okanye i-plasma.Yenzelwe ukuba isetyenziswe kwiilabhoratri zeklinikhi zokuxilongwa kunye nokulawulwa kwezigulane ezinxulumene nokusuleleka kwi-Human Cytomegalovirus.I-HCMV yenye yezona zizathu zibalulekileyo zokuzalwa kakubi ebantwini.Usulelo oluphambili okanye olutsha oluphindaphindiweyo kwabasetyhini abakhulelweyo lunokubangela izifo ze-intrauterine okanye i-perinatal kwiintsana ezisanda kuzalwa.I-80% yabantwana abakhulelweyo abasuleleke nge-HCMV ngexesha lokukhulelwa okuphambili banokuhlakulela iimpawu ezifana nokukhubazeka kwengqondo, ukungaboni kakuhle, kunye nokulahlekelwa kwindlebe.Usulelo olusisiseko lwe-HCMV luneziphumo ezibi kwaye ezibeka ubomi esichengeni kwizigulana ezigogekileyo, kwaye izinga lokufa kwenyumoniya yangaphakathi ngenxa yosulelo lweHCMV kumongo wethambo liphezulu ukuya kuma-75%.Kutshanje, iHCMV ifunyenwe ingomnye wemithombo yosulelo olungenelelayo kwizigulana zikaGawulayo.

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Umgaqo

Le khithi ibhaqa i-antibody ye-Human Cytomegalovirus IgM (HCMV-IgM) kwi-serum yabantu okanye iisampulu zeplasma, imicu ye-polystyrene microwell iqatywe ngezilwa-buhlungu ezijoliswe kwiprotein ye-immunoglobulin M yabantu (itsheyina le-anti-µ).Emva kokuqala ukongeza i-serum okanye iisampuli ze-plasma eziza kuhlolwa, ii-antibodies ze-IgM kwi-specimen zingabanjwa, kunye nezinye izinto ezingabotshwanga (kubandakanywa ne-IgG antibodies) ziya kususwa ngokuhlamba.Kwinqanaba lesibini, i-HRP (i-horseradish peroxidase) -i-antigens edibeneyo iya kusabela ngokukodwa kuphela ngee-antibodies ze-HCMV IgM.Emva kokuhlamba ukususa i-HRP-conjugate engabotshwanga, izisombululo ze-chromogen zongezwa kumaqula.Phambi kwe (anti- µ) -(HCMV-lgM) -(HCMV Ag-HRP) immunocomplex, emva kokuhlamba ipleyiti, i-substrate ye-TMB yongezwa kuphuhliso lombala, kwaye i-HRP edityaniswe kwi-complex ibangela ukusabela komphuhlisi wombala velisa into eluhlaza, yongeza i-50 µI yeStop Solution, kwaye ujike ube tyheli.Ubukho be-absorbection ye-antibody ye-HCMV-IgM kwisampulu inqunywe ngumfundi we-microplate.

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Ukucaciswa kweMveliso

Umgaqo	Uvavanyo lwe-Enzyme olunxibelelene ne-immunosorbent
Uhlobo	Indlela yokubamba
Isatifikethi	NMPA
Umzekelo	Iserum yabantu / iplasma
Inkcazo	48T / 96T
Ubushushu bokugcina	2-8℃
Beka ubomi kwishelufa	Iinyanga ezili-12