Intsholongwane yeRubella IgM ELISA Kit

Rubella virus IgM ELISA Kit Featured Image

Inkcazelo emfutshane:

I-Rubella virus IgM (RV-IgM) I-ELISA Kit yi-enzyme-edityaniswe ne-immunosorbent assay yokufumanisa umgangatho we-IgM-class antibodies kwi-virus ye-Rubella kwi-serum yabantu okanye i-plasma.I-Anti-rubella virus Izilwa-buhlungu ze-IgM kwiserum okanye kwiplasma yabantu zinxulunyaniswa ngokusondeleyo nosulelo olubukhali lwentsholongwane, kwaye izilwa-buhlungu ze-IgM ezikhethekileyo ze-rubella zezinye zezalathi ezinovakalelo nezikhethekileyo zoxilongo lwaselabhoratri.Ukufunyaniswa kwe-rubella virus IgM ibalulekile kwi-eugenics kunye nesikhokelo seklinikhi.Uvavanyo lwe-antibody lwe-Rubella lwe-IgM akufuneki lusetyenziselwe ukuhlolwa kwangaphambi kokubeleka kwabantu abangenazimpawu, kwaye iziphumo zovavanyo akufuneki zisetyenziswe zodwa njengesiseko sokuphelisa ukukhulelwa.

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Umgaqo

Le khithi ibhaqa i-antibody ye-Rubella virus IgM (RV-IgM) kwiserum yabantu okanye iisampulu zeplasma, imicu ye-polystyrene microwell iqatywe ngezilwa-buhlungu ezijoliswe kwiprotein ye-immunoglobulin M yabantu (itsheyina le-anti-µ).Emva kokuqala ukongeza i-serum okanye iisampuli ze-plasma eziza kuhlolwa, ii-antibodies ze-IgM kwi-specimen zingabanjwa, kunye nezinye izinto ezingabotshwanga (kubandakanywa ne-IgG antibodies) ziya kususwa ngokuhlamba.Kwinqanaba lesibini, i-HRP (i-horseradish peroxidase) -i-antigens edibeneyo iya kusabela ngokukodwa kuphela nge-antibodies ye-RV IgM.Emva kokuhlamba ukususa i-HRP-conjugate engabotshwanga, izisombululo ze-chromogen zongezwa kumaqula.Phambi kwe (anti- µ) -(RV-lgM) -(RV Ag-HRP) immunocomplex, emva kokuhlamba ipleyiti, i-substrate ye-TMB yongezwa kuphuhliso lombala, kunye ne-HRP eqhagamshelwe kwi-complex ivuselela ukusabela komphuhlisi wombala velisa into eluhlaza, yongeza i-50 µI yeStop Solution, kwaye ujike ube tyheli.Ubukho be-absorbection ye-antibody ye-RV-IgM kwisampulu yagqitywa ngumfundi we-microplate.

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Ukucaciswa kweMveliso

Umgaqo	Uvavanyo lwe-Enzyme olunxibelelene ne-immunosorbent
Uhlobo	Indlela yokubamba
Isatifikethi	NMPA
Umzekelo	Iserum yabantu / iplasma
Inkcazo	48T / 96T
Ubushushu bokugcina	2-8℃
Beka ubomi kwishelufa	Iinyanga ezili-12