Herpes Simplex II IgM ELISA Kit
Umgaqo
Le khithi ibona i-Herpes Simplex virus II IgM antibody (HSV2-IgM) kwi-serum yabantu okanye iisampulu zeplasma, imicu ye-polystyrene microwell ifakwe ngaphambili kunye nezilwa-buhlungu ezijoliswe kwiprotheni yomntu ye-immunoglobulin M (anti-µ chain).Emva kokuqala ukongeza i-serum okanye iisampuli ze-plasma eziza kuhlolwa, ii-antibodies ze-IgM kwi-specimen zingabanjwa, kunye nezinye izinto ezingabotshwanga (kubandakanywa ne-IgG antibodies) ziya kususwa ngokuhlamba.Kwinqanaba lesibini, i-HRP (i-horseradish peroxidase) -i-antigens edibeneyo iya kusabela ngokukodwa kuphela nge-HSV2 IgM antibodies.Emva kokuhlamba ukususa i-HRP-conjugate engabotshwanga, izisombululo ze-chromogen zongezwa kumaqula.Phambi kwe (anti-µ)-(HSV2-lgM)-(HSV2 Ag-HRP) immunocomplex, emva kokuhlamba ipleyiti, i-substrate ye-TMB yongezwa kuphuhliso lombala, kwaye i-HRP edityaniswe kwi-complex ibangela ukusabela komphuhlisi wombala velisa into eluhlaza, yongeza i-50 µ I yeStop Solution, kwaye ujike ube tyheli.Ubukho bokuthatha i-antibody ye-HSV2-IgM kwisampulu yagqitywa ngumfundi we-microplate.
Iimpawu zeMveliso
Uvakalelo oluphezulu, ukuchaneka kunye nokuzinza
Ukucaciswa kweMveliso
Umgaqo | Uvavanyo lwe-Enzyme olunxibelelene ne-immunosorbent |
Uhlobo | Indlela yokubamba |
Isatifikethi | NMPA |
Umzekelo | Iserum yabantu / iplasma |
Inkcazo | 48T / 96T |
Ubushushu bokugcina | 2-8℃ |
Beka ubomi kwishelufa | Iinyanga ezili-12 |
Ukuodola Ulwazi
Igama lemveliso | Pakisha | Umzekelo |
Herpes Simplex II IgM ELISA Kit | 48T / 96T | Iserum yabantu / iplasma |