Intsholongwane yeHepatitis D IgM ELISA Kit

Inkcazelo emfutshane:

Intsholongwane ye-Hepatitis D IgM ELISA Kit yi-enzyme-edityaniswe ne-immunosorbent assay yokukhangela umgangatho we-IgM-class antibodies kwi-virus ye-hepatitis D kwi-serum yabantu okanye i-plasma.Yenzelwe ukuba isetyenziswe kwiilabhoratri zeklinikhi zokuxilongwa kunye nokulawulwa kwezigulane ezinxulumene nosulelo lwentsholongwane ye-hepatitis D.I-anti-HDV-IgM entle ibonisa usulelo oluqatha okanye lwamva nje lwe-HDV;i-co-infection kunye ne-HDV / HBV idibaniswa ne-anti-HDV-IgM engapheliyo;usulelo oluthe kratya lunxulunyaniswa ne-anti-HDV-IgM eqhubekayo;ngoko ke, uvavanyo lwe-anti-HDV-IgM lunexabiso elibalulekileyo lokuxilonga.Amajoni omzimba eAnti-HDV-IgM anokwahlula phakathi kosulelo lwamva nje okanye lwangaphambili, ukosuleleka ngokubambisana kunye nosulelo oludlulayo, kunye namanqaku aphezulu aqhubekayo e-anti-HDV-IgG kunye ne-IgM aguquguquka ngokuphindaphindiweyo, ezi zizalathisi ezibalulekileyo zosulelo lwe-HDV engapheliyo, ngoko ke ukubonwa kwe-anti- I-HDV-IgM ibalulekile ekuxilongeni nasekuxilongweni kosulelo lwe-HDV.

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Iithegi zeMveliso

Umgaqo

Le khithi ibhaqa intsholongwane ye-hepatitis D i-antibody ye-IgM (i-HDV-IgM) kwi-serum yabantu okanye iisampulu ze-plasma, imicu ye-polystyrene microwell ifakwe ngaphambili kunye nezilwa-buhlungu ezijoliswe kwiiprotheni ze-immunoglobulin M (anti-μ chain).Emva kokuqala ukongeza i-serum okanye iisampuli ze-plasma eziza kuhlolwa, ii-antibodies ze-IgM kwi-specimen zingabanjwa, kunye nezinye izinto ezingabotshwanga (kubandakanywa ne-IgG antibodies) ziya kususwa ngokuhlamba.Kwinqanaba lesibini, i-HRP (i-horseradish peroxidase) -i-antigens edibeneyo iya kusabela ngokukodwa kuphela nge-antibodies ye-HDV IgM.Emva kokuhlamba ukususa i-HRP-conjugate engabotshwanga, izisombululo ze-chromogen zongezwa kumaqula.Phambi (anti-μ) - (HDV-IgM) - (HDV Ag-HRP) immunocomplex, emva kokuhlamba ipleyiti, substrate TMB yongezwa kuphuhliso lombala, kunye HRP edityaniswe entsonkothileyo catalyzes ukusabela umphuhlisi umbala ukuba velisa into eluhlaza, yongeza i-50μl yeStop Solution, kwaye ujike ube tyheli.Ubukho be-absorbection ye-antibody ye-HDV-IgM kwisampulu inqunywe ngumfundi we-microplate.

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Uvakalelo oluphezulu, ukuchaneka kunye nokuzinza

Ukucaciswa kweMveliso

Umgaqo	Uvavanyo lwe-Enzyme olunxibelelene ne-immunosorbent
Uhlobo	Indlela yokubamba
Isatifikethi	NMPA
Umzekelo	Iserum yabantu / iplasma
Inkcazo	48T / 96T
Ubushushu bokugcina	2-8℃
Beka ubomi kwishelufa	Iinyanga ezili-12