Enterovirus 71(EV71) IgM ELISA Kit

Inkcazelo emfutshane:

I-Enterovirus 71 IgM (EV71-IgM) ikhithi ye-ELISA yi-enzyme-exhunywe kwi-immunosorbent assay yokufumanisa umgangatho we-IgM-class antibodies kwi-Enterovirus 71 kwi-serum yabantu okanye i-plasma.Yenzelwe ukuba isetyenziswe kwiilabhoratri zeklinikhi zokuxilongwa kunye nokulawulwa kwezigulana ezinxulumene nosulelo lwe-Enterovirus 71.

I-enterovirus yabantu 71 (EV71), ilungu elitsha le-Enteroviridae, yimbangela eqhelekileyo yesandla, unyawo kunye nesifo somlomo (HFMD) kwaye ngamanye amaxesha ihambelana nezifo ezinzima ze-nervous system.Usulelo ludla ngokukhula ngoJuni nangoJulayi.Usulelo nge-EV71 lunokuba lungabonakali okanye lunokubangela urhudo kunye nerhashalala.Abantwana abangaphantsi kweminyaka emihlanu basemngciphekweni omkhulu wokufumana usulelo oluyingozi kunabantu abadala kunye nabantwana abadala.

I-EV71 idluliselwa ukusuka komnye umntu ukuya komnye ngokudibana ngokuthe ngqo nangokusuleleka kwezandla okanye izinto ezinobulongwe obusulelekileyo.Ukuphuma kweempumlo nasemqaleni, amathe okanye ulwelo oluphuma kumadyunguza nako kunokusasaza intsholongwane.

I-EV71 inokubekwa yodwa kwaye ibonwe emqaleni nakwizitulo zesitulo kusetyenziswa uvavanyo lwe-PCR.I-Viral RNA ifunyenwe kulwelo lwe-vesicle yesikhumba, igazi kunye nomchamo.Ukuxilongwa kwangaphambili kwaye kulula kunokufezekiswa kusetyenziswa i-EV71-specific serological assays, kubandakanya novavanyo oluthile lwe-antibody ye-IgM.

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

Umgaqo

Le khithi ibhaqa i-antibody ye-Enterovirus 71 IgM (EV71-IgM) kwi-serum yabantu okanye iisampulu zeplasma, imicu ye-polystyrene microwell ifakwe ngaphambili kunye ne-antibodies ejoliswe kwi-protein ye-immunoglobulin M yabantu (i-anti-μ chain). ihlolwe, ii-antibodies ze-IgM kwi-specimen zingabanjwa, kunye nezinye izinto ezingabotshwanga (kubandakanywa ne-IgG antibodies ezithile) ziya kususwa ngokuhlamba.Kwinqanaba lesibini, i-HRP (i-horseradish peroxidase) -i-antigens edibeneyo iya kusabela ngokukodwa kuphela nge-EV71 IgM antibodies.Emva kokuhlamba ukususa i-HRP-conjugate engabotshwanga, izisombululo ze-chromogen zongezwa kumaqula.Phambi kwe (anti-μ) - (EV71-IgM) - (EV71-Ag-HRP) immunocomplex, emva kokuhlamba ipleyiti, i-substrate TMB yongezwa kuphuhliso lombala, kunye ne-HRP eqhagamshelwe kwi-complex ibangela ukusabela komphuhlisi wombala. ukuvelisa into eluhlaza, yongeza i-50μL yeStop Solution, kwaye ujike ube tyheli.Ubukho bokutsalwa kwe-antibody ye-EV71-IgM kwisampulu yagqitywa ngumfundi we-microplate.

Iimpawu zeMveliso

Uvakalelo oluphezulu, ukuchaneka kunye nokuzinza

Ukucaciswa kweMveliso

Umgaqo	Uvavanyo lwe-Enzyme olunxibelelene ne-immunosorbent
Uhlobo	Indlela yokubamba
Isatifikethi	CE
Umzekelo	Iserum yabantu / iplasma
Inkcazo	48T / 96T
Ubushushu bokugcina	2-8℃
Beka ubomi kwishelufa	Iinyanga ezili-12