Intsholongwane yeHepatitis E IgM ELISA Kit

Hepatitis E virus IgM ELISA Kit Featured Image

Inkcazelo emfutshane:

Intsholongwane ye-Hepatitis E IgM Test Cassette isetyenziselwa ukufunyanwa ngokomgangatho we-Hepatitis E Virus IgM antibodies kwi-serum yabantu, i-plasma (EDTA, i-heparin, i-sodium citrate) okanye igazi elipheleleyo (EDTA, i-heparin, i-sodium citrate).Uvavanyo luya kusetyenziswa njengoncedo ekuxilongeni i-hepatitis yentsholongwane, ebangelwa yi-Hepatitis E Virus.

Intsholongwane ye-Hepatitis E (HEV) yintsholongwane ye-RNA engaguqukiyo, enomtya omnye esasazwa ngendlela ye-fecal-oral, utofelo-gazi kunye mhlawumbi ne-fetal-fetal.Ukosulelwa yi-HEV kubangela i-acute sporadic kunye ne-epidemic viral Hepatitis kwaye yenza izifo zesibindi ezibukhali okanye ezincinci ezifana ne-Hepatitis A. Nangona kukho iigenotypes ezine ze-HEV, kukho i-serotype enye kuphela.

Usulelo lwe-HEV ebantwini luvelisa izilwa-buhlungu ze-IgM, i-IgA kunye ne-IgG.I-HEV-IgM kunye ne-HEV-IgA positivity luphawu losulelo olubukhali okanye lwamva nje lwe-HEV.Nokuba i-anti-HEV-IgM kunye ne-anti-HEV-IgA zilungile kwenye okanye zombini, zibonisa usulelo lwamva nje lwe-HEV.Ubukho bosulelo lwe-HEV lwakutsha nje, ludityaniswe nokusebenza kwesibindi, lunokusetyenziselwa ukufumanisa ukuba usulelo lubukhali okanye lwakutshanje.Ubukho bosulelo lwe-HEV esibindini bunokusetyenziselwa ukufumanisa ukuba isifo si-acute hepatitis E okanye ukuchacha kwi-hepatitis E ebukhali.

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Umgaqo

Le khithi ibhaqa i-antibody ye-hepatitis E ye-IgM (HEV-IgM) kwi-serum yabantu okanye iisampulu zeplasma, imicu ye-polystyrene microwell iqatywe ngaphambili kunye nezilwa-buhlungu ezijoliswe kwiiprotheni zabantu ze-immunoglobulin M (anti-μ chain).Emva kokuqala ukongeza i-serum okanye iisampuli ze-plasma eziza kuhlolwa, ii-antibodies ze-IgM kwi-specimen zingabanjwa, kunye nezinye izinto ezingabotshwanga (kubandakanywa ne-IgG antibodies) ziya kususwa ngokuhlamba.Kwinqanaba lesibini, i-HRP (i-horseradish peroxidase) -i-antigens edibeneyo iya kusabela ngokukodwa kuphela nge-HEV IgM antibodies.Emva kokuhlamba ukususa i-HRP-conjugate engabotshwanga, izisombululo ze-chromogen zongezwa kumaqula.Phambi (anti-μ) - (HEV-IgM) - (HEV Ag-HRP) immunocomplex, emva kokuhlamba ipleyiti, substrate TMB yongezwa kuphuhliso lombala, kunye HRP edityaniswe entsonkothileyo catalyzes ukusabela umphuhlisi umbala ukuba velisa into eluhlaza, yongeza i-50μl yeStop Solution, kwaye ujike ube tyheli.Ubukho bokutsalwa kwe-antibody ye-HEV-IgM kwisampulu yagqitywa ngumfundi we-microplate.

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Ukucaciswa kweMveliso

Umgaqo	Uvavanyo lwe-Enzyme olunxibelelene ne-immunosorbent
Uhlobo	Indlela yokubamba
Isatifikethi	CE
Umzekelo	Iserum yabantu / iplasma
Inkcazo	48T / 96T
Ubushushu bokugcina	2-8℃
Beka ubomi kwishelufa	Iinyanga ezili-12