Uvavanyo lokuxilonga lwe-TB-IGRA

TB-IGRA Uvavanyo loDiagnostic Featured Image

Inkcazelo emfutshane:

Uvavanyo lokuxilonga lwe-TB-IGRA, olukwabizwa ngokuba yi-Interferon Gamma Release Assay, yi-ELISA yokufumanisa ubungakanani be-Interferon Gamma (IFN-γ) esabela kwi-in vitro stimulation yi-Mycobacterium Tuberculosis antigens kwiisampuli zegazi lomntu.I-TB-IGRA ilinganisa amandla omzimba womntu kwi-Mycobacterium Tuberculosis.Uvavanyo lwenzelwe ukusetyenziswa njengoncedo ekufunyanisweni kosulelo lwe-TB kubandakanywa ukosuleleka kweSifo sePhepha esifihlakeleyo kunye nesifo seSifo sePhepha.

Ukusabela komzimba okubangelwa yi-Mycobacterium tuberculosis ikakhulu yimpendulo yeselula.Emva kokusuleleka kwisifo sephepha seMycobacterium, umzimba uvelisa iiseli ezithile zememori T ezijikeleza kwigazi elijikelezayo.Umlinganiselo we-IFN-γ ngokuchasene ne-Mycobacterium tuberculosis ibonakaliswe njengendlela esebenzayo yokubona usulelo lwe-TB (zombini ezifihlakeleyo kunye nesebenzayo), ebizwa ngokuba yi-IFN-γ in vitro release assay (IGRA).Umahluko omkhulu kuvavanyo lwe-tuberculin (TST) kukuba i-IGRA ikhetha ii-antigens ezithile ezikhoyo kuphela kwi-Mycobacterium tuberculosis kodwa azikho kwi-BCG kunye neyona mycobacteria engeyiyo i-tuberculous.

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

Umgaqo

Ikhithi ithatha i-interferon-γ yokukhutshwa kovavanyo lwe-Mycobacterium tuberculosis (TB-IGRA) ukulinganisa ubunzulu bempendulo ye-immune yeselula elawulwa yi-Mycobacterium tuberculosis specific antigen.
Uvavanyo lwe-Enzyme-linked immunosorbent kunye nomgaqo wesangweji ye-antibody ephindwe kabini.
• Ii-microplates ziqatywe ngaphambili ngamajoni alwa ne-IFN-γ.
• Iisampulu eziza kuvavanywa zongezwa kwiqula le-antibody coated microplate, emva koko i-horseradish peroxidase (HRP)-conjugated anti-IFN-γ antibodies zongezwa kwiqula elichaphazelekayo.
• I-IFN-γ, ukuba ikhona, iya kwenza i-sandwich complex kunye ne-anti-IFN-γ antibodies kunye ne-HRP-conjugated anti-IFN-γ antibodies.
• Umbala uya kuphuhliswa emva kokongeza izisombululo ze-substrate, kwaye uya kutshintsha emva kokongeza izisombululo zokumisa.I-absorbence (OD) ilinganiswa nomfundi we-ELISA.
• I-IFN-γ yoxinaniso kwisampulu ihambelana ne-OD emiselweyo.

Iimpawu zeMveliso

Uphononongo olusebenzayo lwe-ELISA losulelo lwe-TB olufihlakeleyo nolusebenzayo

Akukho kuphazamiseka kwesitofu sokugonya se-BCG

Ukucaciswa kweMveliso

Umgaqo	Uvavanyo lwe-Enzyme olunxibelelene ne-immunosorbent
Uhlobo	Indlela yeSandwich
Isatifikethi	CE, NMPA
Umzekelo	Igazi elipheleleyo
Inkcazo	48T (bona iisampuli ze-11);96T (bona iisampulu ezingama-27)
Ubushushu bokugcina	2-8℃
Beka ubomi kwishelufa	Iinyanga ezili-12