Enterovirus 71(EV71) IgG ELISA Kit

Inkcazelo emfutshane:

I-Human Enterovirus 71 IgG ELISA Kit yi-enzyme-linked immunosorbent assay yokukhangela umgangatho we-IgG-class antibodies kwi-Enterovirus 71 kwi-serum yabantu okanye i-plasma.Yenzelwe ukuba isetyenziswe kwiilabhoratri zeklinikhi zokuxilongwa kunye nokulawulwa kwezigulana ezinxulumene nosulelo lwe-Enterovirus 71.

Ukubonakaliswa kweklinikhi ephambili ye-EV71 usulelo sisifo sesandla, unyawo kunye nomlomo.Abanye abantwana abanosulelo lwe-EV71 baba ne-herpetic pharyngitis, kwaye kwiimeko ezinzima, i-encephalitis yentsholongwane, i-encephalomyelitis yentsholongwane, i-pulmonary edema kunye ne-pulmonary hemorrhage.

Ukuxilongwa okuthe ngqo kokusuleleka kwe-EV71 kuxhomekeke ekufumaneni i-EV71-RNA, i-EV71-IgM kunye ne-EV71-IgG ye-antibodies kwi-serum, okanye i-EV71-RNA kwi-swab specimens.

I-EV71-IgM isalathiso esibalulekileyo sosulelo oluphambili okanye lwakutsha nje kwaye luququzelele ukufunyanwa kwangoko kunye nonyango lwe-EV71 usulelo.I-EV71-IgG isalathiso esibalulekileyo sokuxilongwa kosulelo kwaye ingasetyenziselwa uphando lwe-epidemiological kunye novavanyo lokugonywa.Ingasetyenziselwa uphando lwe-epidemiological kunye novavanyo lwesiphumo sogonyo.Utshintsho lweempawu ze-antibody kwigazi elifunyenweyo kunye negazi elibukhali nako kunokumisela usulelo lwe-EV71, kwaye ukuba i-titer ye-antibody ye-serum yonyuka ngokwejometri ngo-4 kwixesha elifunyenweyo xa kuthelekiswa nexesha elibukhali, inokugwetywa njenge-EV71 ebonisa usulelo.

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Iithegi zeMveliso

Umgaqo

Le kit ibona i-Enterovirus 71 IgG antibody (EV71-IgG) kwi-serum yabantu okanye iisampulu zeplasma, imicu ye-polystyrene microwell ifakwe ngaphambili nge-Enterovirus 71 antigen.Emva kokuqala ukongeza i-serum okanye iisampuli ze-plasma eziza kuhlolwa, ii-antibodies ezihambelanayo (EV71-IgG-Ab & ezinye ze-IgM-Ab) ezikhoyo kwiimpawu zesigulane zibophelela kwi-antigens kwisigaba esiqinileyo, kunye nezinye izinto ezingabonakaliyo ziya kususwa ngokuhlamba.Kwinqanaba lesibini, i-HRP (i-horseradish peroxidase) -i-conjugated anti-human IgG iya kusabela ngokukodwa kuphela nge-EV71 IgG antibodies.Emva kokuhlamba ukususa i-HRP-conjugate engabotshwanga, izisombululo ze-chromogen zongezwa kumaqula.Phambi kwe (EV71 Ag) - (EV71-IgG) - (anti-human IgG-HRP) immunocomplex, emva kokuhlamba ipleyiti, i-substrate TMB yongezwa kuphuhliso lombala, kunye ne-HRP edityaniswe kwi-complex ibangela ukusabela komphuhlisi wombala. ukwenza into eluhlaza, yongeza i-50μL yeStop Solution, kwaye ujike ube tyheli.Ubukho bokutsalwa kwe-antibody ye-EV71-IgG kwisampulu yagqitywa ngumfundi we-microplate.

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Ukucaciswa kweMveliso

Umgaqo	Uvavanyo lwe-Enzyme olunxibelelene ne-immunosorbent
Uhlobo	Indlela engathanga ngqo
Isatifikethi	CE
Umzekelo	Iserum yabantu / iplasma
Inkcazo	48T / 96T
Ubushushu bokugcina	2-8℃
Beka ubomi kwishelufa	Iinyanga ezili-12