Mycoplasma Pneumonia IgM ELISA Kit
Umgaqo
Le khithi ibhaqa i-Mycoplasma Pneumoniae IgM (MP-IgM) kwi-serum yabantu okanye iisampulu zeplasma, imicu ye-polystyrene microwell ifakwe ngaphambili kunye ne-antibodies ejoliswe kwiiprotheni zomntu ze-immunoglobulin M (i-anti-μ chain). , ii-antibodies ze-IgM kwi-specimen zingabanjwa, kunye nezinye izinto ezingabonakaliyo (kubandakanywa ne-antibodies ezithile ze-IgG) ziya kususwa ngokuhlamba.Kwinqanaba lesibini, i-HRP (i-horseradish peroxidase) -i-antigens edibeneyo iya kusabela ngokukodwa kuphela nge-MP IgM antibodies.Emva kokuhlamba ukususa i-HRP-conjugate engabotshwanga, izisombululo ze-chromogen zongezwa kumaqula.Phambi kwe (anti-μ) - (MP-IgM) - (MP-Ag-HRP) immunocomplex, emva kokuhlamba ipleyiti, i-substrate ye-TMB yongezwa kuphuhliso lombala, kunye ne-HRP eqhagamshelwe kwi-complex ibangela ukusabela komphuhlisi wombala. ukuvelisa into eluhlaza, yongeza i-50μl yeStop Solution, kwaye ujike ube tyheli.Ubukho bokutsalwa kwe-antibody ye-MP-IgM kwisampulu yagqitywa ngumfundi we-microplate.
Iimpawu zeMveliso
Uvakalelo oluphezulu, ukuchaneka kunye nokuzinza
Ukucaciswa kweMveliso
Umgaqo | Uvavanyo lwe-Enzyme olunxibelelene ne-immunosorbent |
Uhlobo | Indlela yokubamba |
Isatifikethi | NMPA |
Umzekelo | Iserum yabantu / iplasma |
Inkcazo | 96T |
Ubushushu bokugcina | 2-8℃ |
Beka ubomi kwishelufa | Iinyanga ezili-12 |
Ukuodola Ulwazi
Igama lemveliso | Pakisha | Umzekelo |
Mycoplasma Pneumoniae IgM ELISA Kit | 96T | Iserum yabantu / iplasma |