Intsholongwane yemasisi (MV) IgG ELISA Kit

Inkcazelo emfutshane:

Intsholongwane yemasisi (MV) IgG ELISA Kit luvavanyo lwe-enzyme-linked immunosorbent ukuze kufunyanwe umgangatho we-IgG-class antibodies kwintsholongwane yeMasisi kwiserum yomntu okanye kwiplasma.Yenzelwe ukuba isetyenziswe kwiilabhoratri zeklinikhi ekuxilongeni nasekulawuleni izigulana ezinxulumene nosulelo lweNtsholongwane yeMasisi.

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Umgaqo

Le khithi ibhaqa intsholongwane yeMasisi i-IgG antibody (MV-IgG) kwiserum yabantu okanye iisampulu zeplasma, imicu ye-polystyrene microwell iqatywe nge-antigen yeMasisi.Emva kokuqala ukongeza i-serum okanye iisampuli ze-plasma eziza kuhlolwa, ii-antibodies ezithile ezihambelanayo (MV-IgG-Ab & ezinye ze-IgM-Ab) ezikhoyo kwiimpawu zesigulane zibophelela kwii-antigens kwisigaba esiqinileyo, kunye nezinye izinto ezingabonakaliyo ziya kususwa ngokuhlamba.Kwinqanaba lesibini, i-HRP (i-horseradish peroxidase) -i-conjugated anti-human IgG iya kusabela ngokukodwa kuphela ngee-antibodies ze-MV IgG.Emva kokuhlamba ukususa i-HRP-conjugate engabotshwanga, izisombululo ze-chromogen zongezwa kumaqula.Phambi (MV Ag) - (MV-IgG) - (anti-human IgG-HRP) immunocomplex, emva kokuhlamba ipleyiti, substrate TMB yongezwa uphuhliso lombala, kunye HRP eqhagamshelwe entsonkothileyo catalyzes ukusabela umphuhlisi umbala. ukuvelisa into eluhlaza okwesibhakabhaka, yongeza i-50μl ye-Stop Solution, kwaye ijike ibe tyheli.Ubukho be-absorption ye-antibody ye-MV-IgG kwisampulu inqunywe ngumfundi we-microplate.

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Uvakalelo oluphezulu, ukuchaneka kunye nokuzinza

Ukucaciswa kweMveliso

Umgaqo	Uvavanyo lwe-Enzyme olunxibelelene ne-immunosorbent
Uhlobo	Indlela Engathanga ngqo
Isatifikethi	NMPA
Umzekelo	Iserum yabantu / iplasma
Inkcazo	48T / 96T
Ubushushu bokugcina	2-8℃
Beka ubomi kwishelufa	Iinyanga ezili-12