Anti-Zona Pellucida (ZP) Antibody ELISA Kit

I-Anti-Zona Pellucida (ZP) i-Antibody ELISA Kit ebonisiweyo

Inkcazelo emfutshane:

Le mveliso yenzelwe ukufunyaniswa komgangatho we-in vitro we-zona pellucida (ZP) amanqanaba e-antibody kwiserum yomntu. I-zona pellucida, imatrix ekhethekileyo ye-extracellular ejikeleze i-oocyte, idlala indima ebalulekileyo ekuqapheliseni amadlozi, ukubopha, kunye nokuchumisa, kunye nophuhliso lwangethuba lwe-embryonic.

Izilwa-buhlungu ze-ZP zi-autoantibodies ezijolise kwii-antigens ze-zona pellucida. Xa zivela emzimbeni, zinokuzibophelela ngokukodwa kwi-zona pellucida, zithintele intsebenziswano eqhelekileyo phakathi kwedlozi kunye ne-oocyte, ngaloo ndlela ithintele ukuchumisa. Ukongezelela, banokuphazamisana nenkqubo yokufakelwa kwamaqanda achumileyo, enye yezona zizathu ezibalulekileyo zokungakhuli kwe-autoimmune.

Ngokweklinikhi, oku kuchongwa kuyasebenza njengendlela encedisayo yokuxilongwa kwe-autoimmune infertility. Ngokubona izinga le-antibodies ze-ZP kwi-serum yezigulane, inokubonelela ngolwazi oluxabisekileyo lwereferensi yokucacisa izizathu zokungazali kwezinye izigulana ezinezizathu ezingaziwayo, ukunceda oogqirha ukuba benze izicwangciso ezijoliswe kuzo zokuxilonga kunye nonyango.

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

Umgaqo

Le khithi ibhaqa izilwa-buhlungu ze-zona pellucida (ZP-Ab) kwiisampulu zeserum yabantu ngokusekelwe kwindlela engathanga ngqo, kunye ne-zona pellucida ecocekileyo esetyenziswa njenge-antigen yokwambathisa.

Inkqubo yokuvavanya iqala ngokudibanisa isampuli ye-serum kwimithombo yokusabela efakwe ngaphambili kunye ne-antigen, ilandelwa yi-incubation. Ukuba i-ZP-Ab ikhona kwisampulu, iya kubophelela ngokukodwa kwi-coated zona pellucida antigen emaquleni, yenze ii-antigen-antibody complexes ezizinzileyo.

Emva koko, i-enzyme conjugates yongezwa kumaqula. Emva kwenyathelo lesibini lokufukamela, ezi enzyme zidibanisayo zibophelela kwii-antigen-antibody complexes ezikhoyo. Xa isisombululo se-TMB substrate singeniswa, ukusabela kombala kwenzeka phantsi kwesenzo se-catalytic ye-enzyme kwi-complex. Ekugqibeleni, umfundi we-microplate usetyenziselwa ukulinganisa i-absorbence (i-A value), evumela ukuzimisela kwamanqanaba e-ZP-Ab kwisampuli.

Iimpawu zeMveliso

Uvakalelo oluphezulu, ukuchaneka kunye nokuzinza

Ukucaciswa kweMveliso

Umgaqo	Uvavanyo lwe-Enzyme olunxibelelene ne-immunosorbent
Uhlobo	Ngokungathanga ngqoIndlela
Isatifikethi	NIMPA
Umzekelo	Iserum yabantu / iplasma
Inkcazo	48T /96T
Ubushushu bokugcina	2-8℃
Beka ubomi kwishelufa	12iinyanga