I-Anti-Trophoblast Cell Membrane (TA) Antibody ELISA Kit

Inkcazelo emfutshane:

Le mveliso isetyenziselwa ukufunyanwa kwekhwalithi ye-vitro ye-anti-trophoblast cell membrane antibodies kwi-serum yabantu. Iiseli ze-Trophoblast zizinto eziphambili ze-placenta, zidlala indima ebalulekileyo ekufakweni kwe-embryo yokuqala, ukubunjwa kwe-placenta, kunye nokugcina ukunyamezela okuqhelekileyo kwe-immune-fetal.

I-anti-trophoblast cell membrane antibodies zi-autoantibodies ezijolise kwii-antigens kumphezulu weeseli ze-trophoblast. Xa ezi zilwa-buhlungu zibonakala emzimbeni, zinokuhlasela iiseli ze-trophoblast, zonakalise ubume bazo kunye nomsebenzi wazo, ziphazamise ukufakwa kwe-embryo ngokuqhelekileyo, kwaye ziphazamise ibhalansi yokuzivikela phakathi komama nomntwana. Oku kunokukhokelela ekungaphumelelini kokufakelwa, ukulahleka kokukhulelwa kwangoko, okanye ezinye iingxaki zokuzala, zibe ngunobangela wokungachumi kwe-autoimmune.

Ngokweklinikhi, oku kuchongwa kusebenza njengesixhobo esincedisayo sokuxilongwa kwe-autoimmune infertility. Inceda ukuchonga ukuba umonakalo we-immune kwiiseli ze-trophoblast ubandakanyeka kwi-pathogenesis ye-infertility, ukubonelela ngolwazi olubalulekileyo lwereferensi kwiiklinikhi ukucacisa izizathu zokungabikho komntwana kunye nokuphuhlisa izicwangciso ezifanelekileyo zonyango.

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

Umgaqo

Le kit ibona i-trophoblast cell membrane antibodies (TA-Ab) kwiisampuli ze-serum yabantu ngokusekelwe kwindlela engathanga ngqo, kunye ne-membrane yeseli ye-trophoblast ecocekileyo esetyenziswa njenge-antigen yokwambathisa.

Inkqubo yokuvavanya iqala ngokudibanisa isampuli ye-serum kwimithombo yokusabela efakwe ngaphambili kunye ne-antigen, ilandelwa yi-incubation. Ukuba i-TA-Ab ikhona kwisampulu, iya kubophezela ngokukodwa kwi-coated trophoblast cell membrane antigens emigodini, yenze i-antigen-antibody complexes ezinzileyo.

Emva kokususa amacandelo angabonakaliyo ngokuhlamba ukuqinisekisa ukuchaneka kokufumanisa, i-enzyme conjugates yongezwa kumaqula. I-incubation yesibini ivumela ezi zidibaniselwano ze-enzyme ukuba zibophe kwii-antigen-antibody complexes ezikhoyo. Xa isisombululo se-TMB substrate singeniswa, i-enzyme kwi-complex ivuselela ukusabela nge-TMB, ivelise utshintsho olubonakalayo lombala. Ekugqibeleni, umfundi we-microplate ulinganisa i-absorbence (i-A value), esetyenziselwa ukumisela inqanaba le-TA-Ab kwisampuli.

Iimpawu zeMveliso

Uvakalelo oluphezulu, ukuchaneka kunye nokuzinza

Ukucaciswa kweMveliso

Umgaqo	Uvavanyo lwe-Enzyme olunxibelelene ne-immunosorbent
Uhlobo	Ngokungathanga ngqoIndlela
Isatifikethi	NIMPA
Umzekelo	Iserum yabantu / iplasma
Inkcazo	48T /96T
Ubushushu bokugcina	2-8℃
Beka ubomi kwishelufa	12iinyanga