Ikiti yoVavanyo lwe-Anti-SARS-CoV-2 (ELISA)

I-Anti-SARS-CoV-2 iNeutralizing Antibody Test Kit (ELISA) Umfanekiso okhoyo

Inkcazelo emfutshane:

Le mveliso isebenzisa i-enzyme edityaniswe ne-immunosorbent assay ukuze ibone ngokusemgangathweni naziphi na izilwa-buhlungu kwi-serum kunye ne-plasma enokuthi ithintele ukusebenzisana kwe-RBD-ACE2, kwaye le ndlela ayikhawulelwanga yisampuli yeentlobo zegenera kunye ne-antibody subclasses.Izilwa-buhlungu ezingathathi hlangothi zinokuvala i-viral spike glycoprotein receptor binding region (RBD) ekubeni idibane ne-cell surface receptor i-angiotensin-converting enzyme-2 (ACE2).

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

Umgaqo

Ikhithi isebenzisa umgaqo wovavanyo lokhuphiswano ukufumana ii-antibodies ezingathinteliyo ngokuchasene ne-SARS-CoV-2 kwi-serum okanye iisampulu zeplasma.Okokuqala, isampuli kufuneka ivavanywe, ulawulo olulungileyo, kunye nolawulo olubi luxutywe kunye ne-HRP-RBD ukwenzela ukuba i-antibody neutralizing ibophe kwi-HRP-RBD, kwaye emva koko umxube wongezwa kwipleyiti yokubamba ngaphambili ehlanganiswe neprotheni ye-hACE2.I-HRP-RBD engabotshelelwanga kwi-antibody engathathi hlangothi kunye nayo nayiphi na i-antibody ye-HRP-RBD ebotshelelwe kwi-antibody engathathi hlangothi iya kubanjwa kwipleyiti egqunyiweyo, ngelixa i-HRP-RBD i-complex ebotshelelwe kwi-antibody engathathi hlangothi isuswa ngexesha lokuhlanjwa.Isisombululo se-TMB songezelelwa ukuphuhlisa umbala.Ekugqibeleni, isisombululo sokumisa songezwa kwaye impendulo yapheliswa.Ubukho okanye ukungabikho kwe-Anti-SARS-CoV-2 yokungathathi hlangothi kwe-antibody kwisampulu kwagwetywa ngokufunyaniswa (i-A-value) ngumfundi we-microplate.

Iimpawu zeMveliso

Uvakalelo oluphezulu, ukuchaneka kunye nokuzinza

Ukucaciswa kweMveliso

Umgaqo	Uvavanyo lwe-Enzyme olunxibelelene ne-immunosorbent
Uhlobo	Indlela yokhuphiswano
Isatifikethi	CE
Umzekelo	Iserum yabantu / iplasma
Inkcazo	96T
Ubushushu bokugcina	2-8℃
Beka ubomi kwishelufa	Iinyanga ezili-12