I-Anti-Ovarian (AO) I-Antibody ELISA Kit

Inkcazelo emfutshane:

I-ovary iqulethe amaqanda, zona pellucida, iiseli zegranulosa, njl., kwizigaba ezahlukeneyo zophuhliso. Icandelo ngalinye linokuthi lifake ii-anti-ovarian antibodies (AoAb) ngenxa yenkcazo ye-antigen engaqhelekanga. Ukuchitheka kwe-ovarian antigen okubangelwa kukwenzakala kwe-ovarian, usulelo, okanye ukudumba kunokubangela i-AoAb kubantu abanokungasebenzi kakuhle kwamajoni omzimba. I-AoAb iphinda yonakalise i-ovary kwaye yonakalisa umsebenzi wesibeleko kunye ne-placenta, ibangela ubudlolo kunye nokuphuma kwesisu.

I-AoAb yafunyanwa kuqala kwizigulane ezinokusilela kwe-ovarian kwangaphambi kwexesha (POF) kunye ne-amenorrhea yokuqala, edityaniswe nokuphendula kwe-autoimmune. I-AoAb ekuqaleni iyanciphisa ukuchuma kwaye ekugqibeleni ikhokelela ekungaphumeleli kwe-ovarian. Izigulana ezingachumiyo ezine-AoAb eqinisekileyo kodwa akukho-POF inokujongana nemingcipheko ye-POF ephezulu yexesha elizayo, efuna uvandlakanyo logcino lwe-ovarian.

I-AoAb positivity iphezulu kwizigulana ezingachumiyo neziphunyelwa zizisu, nto leyo ebonisa ubudlelwane obusondeleyo. Uphononongo lubonisa ukuba i-AoAb ibangela ubudlolo ngaphezu kokuphunyelwa sisisu. Uphando lwakutsha nje lubhaqa i-AoAb kwizigulana ezininzi ze-PCOS, icebisa ukuba ukudumba kwe-ovarian okubangelwa yi-immune kunye ne-cytokines engaqhelekanga kunokubangela i-PCOS kunye nobudlolo, efuna uphononongo olongezelelweyo.

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

Umgaqo

Le khithi ibona ii-anti-ovarian antibodies (IgG) kwiisampulu zeserum yabantu ngokusekelwe kwindlela engathanga ngqo, kunye ne-antigens ye-ovarian membrane ecocekileyo esetyenziselwa ukugquma kwangaphambili i-microwells.

Inkqubo yokuvavanya iqala ngokudibanisa isampuli serum kwimithombo yokusabela antigen-precoated for incubation. Ukuba ii-anti-ovarian antibodies zikhona kwisampulu, ziya kubophezela ngokuthe ngqo kwi-antigens ye-ovarian membrane antigens kwi-microwells, yenze i-antigen-antibody complexes ezinzileyo. Amacandelo angabotshwanga ayasuswa ukuqinisekisa ukuchaneka kokubhaqwa.

Okulandelayo, i-horseradish peroxidase (HRP)-ebhalwe i-mouse anti-human IgG antibodies zongezwa kumaqula. Emva kokuqandusela okwesibini, ezi zithinteli zibhalwe nge-enzyme zibophelela ngokukodwa kwizilwa-buhlungu ezichasene ne-ovarian kwii-antigen-antibody complexes ezikhoyo, zenza i-"antigen-antibody-enzyme-label" epheleleyo.

Ekugqibeleni, isisombululo se-TMB substrate siyongezwa. I-HRP kwi-complex i-catalyzes i-chemical reaction kunye ne-TMB, ivelisa utshintsho olubonakalayo lombala. I-absorbence (Ixabiso le-Absorbent) yesisombululo sokusabela silinganiswa kusetyenziswa umfundi we-microplate, kwaye ubukho okanye ukungabikho kwe-anti-ovarian antibodies kwisampuli kunqunywe ngokusekelwe kwisiphumo sokuxhamla.

Iimpawu zeMveliso

Uvakalelo oluphezulu, ukuchaneka kunye nokuzinza

Ukucaciswa kweMveliso

Umgaqo	Uvavanyo lwe-Enzyme olunxibelelene ne-immunosorbent
Uhlobo	Ngokungathanga ngqoIndlela
Isatifikethi	NIMPA
Umzekelo	Iserum yabantu / iplasma
Inkcazo	48T /96T
Ubushushu bokugcina	2-8℃
Beka ubomi kwishelufa	12iinyanga