Anti-Islet Cell (ICA) Antibody ELISA Kit

Inkcazelo emfutshane:

Le khithi yenzelwe ukufunyanwa kwekhwalithi ye-vitro yamanqanaba e-anti-islet cell antibody (ICA) kwi-serum yabantu. Ngokweklinikhi, isetyenziswa kakhulu njengesixhobo esincedisayo sokuxilonga kwi-Type 1 Diabetes Mellitus (T1DM).

Izilwa-buhlungu zeeseli ze-Islet zi-autoantibodies ezijolise kwii-antigens kumphezulu okanye ngaphakathi kweeseli ze-islet ze-pancreatic, ngakumbi iiseli ze-β. Ubukho babo buhambelana ngokusondeleyo nomonakalo we-autoimmune kwiiseli ze-islet, eyona nto ibalulekileyo ye-T1DM ye-pathological. Kwinqanaba lokuqala le-T1DM, nangaphambi kokuba iimpawu zeklinikhi ezicacileyo ezifana ne-hyperglycemia zivele, i-ICA inokubonwa rhoqo kwi-serum, iyenze ibe yinto ebalulekileyo yokumakisha kwangaphambi kwesi sifo.

Kubantu abanembali yentsapho yesifo sikashukela okanye abo babonisa iimpawu zangaphambi kwesifo sikashukela, ukufumanisa amanqanaba e-ICA kunceda ukuvavanya umngcipheko wokuphuhlisa i-T1DM. Ukongezelela, kwizigulane ezinezizathu ezingaqondakaliyo ze-hyperglycemia, i-ICA inceda ekuhloleni ukwahlula i-T1DM kwezinye iintlobo zesifo sikashukela, ngaloo ndlela ikhokela ukuqulunqwa kwezicwangciso zonyango ezifanelekileyo. Ngokubeka esweni utshintsho kumanqanaba e-ICA, inokubonelela ngereferensi yokuvavanya ukuqhubela phambili komonakalo we-islet cell kunye nokusebenza kwamanyathelo okungenelela.

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

Umgaqo

Le khithi ibona i-antibodies yeseli ye-islet (ICA) kwiisampuli ze-serum yabantu ngokusekelwe kwindlela engathanga ngqo, kunye ne-antigens ye-islet cell ecocekileyo esetyenziswa njenge-antigen yokwambathisa.

Inkqubo yokuvavanya iqala ngokudibanisa isampuli ye-serum kwimithombo yokusabela efakwe ngaphambili kunye ne-antigen, ilandelwa yi-incubation. Ukuba i-ICA ikhona kwisampulu, iya kubophezela ngokuthe ngqo kwi-antigen cell cell cell antigens emaquleni, yenze i-antigen-antibody complexes ezinzileyo. Amacandelo angabotshwanga asuswe ngokuhlamba ukuze kuqinisekiswe ukuchaneka kweempendulo ezilandelayo.

Emva koko, i-enzyme conjugates yongezwa kumaqula. Emva kwenyathelo lesibini lokufukamela, ezi enzyme zidibanisayo zibophelela kwii-antigen-antibody complexes ezikhoyo. Xa isisombululo se-TMB substrate singeniswa, i-enzyme kwi-complex ivuselela ukusabela nge-TMB, okukhokelela ekutshintsheni kombala obonakalayo. Ekugqibeleni, umfundi we-microplate usetyenziselwa ukulinganisa i-absorbeance (Ixabiso le-A), elivumela ukuzimisela kwamanqanaba e-ICA kwisampuli ngokusekelwe kubukhulu bokuphendula kombala.

Iimpawu zeMveliso

Uvakalelo oluphezulu, ukuchaneka kunye nokuzinza

Ukucaciswa kweMveliso

Umgaqo	Uvavanyo lwe-Enzyme olunxibelelene ne-immunosorbent
Uhlobo	Ngokungathanga ngqoIndlela
Isatifikethi	NIMPA
Umzekelo	Iserum yabantu / iplasma
Inkcazo	48T /96T
Ubushushu bokugcina	2-8℃
Beka ubomi kwishelufa	12iinyanga