I-Anti-Insulin (INS) i-Antibody ELISA Kit

I-Anti-Insulin (INS) ye-Antibody ELISA Kit ebonisiweyo Umfanekiso

Inkcazelo emfutshane:

Le khithi isetyenziselwa ukufumanisa umgangatho we-in vitro we-anti-insulin antibodies kwi-serum yabantu.

Kubantu abaqhelekileyo, ubukho be-insulin antibodies egazini bubenza bathambekele ekuphuhliseni i-Type 1 Diabetes Mellitus (T1DM). I-anti-insulin antibodies inokuveliswa ngenxa yomonakalo we-β-cell, ngoko ke ukufumanisa kwabo kunokusebenza njengophawu lokwenzakala kwe-autoimmune β-cell. Zikwangamanqaku okuqala omzimba avela kubantwana abasengozini enkulu ye-T1DM, kwaye ingasetyenziselwa ukufumanisa kwangaphambili kunye nokuthintela i-T1DM, kunye nokubonelela ngesikhokelo esithile sokuxilongwa kunye nokuxilongwa kwe-T1DM.

Ubukho be-insulin antibodies egazini ngunobangela obalulekileyo wokunganyangeki kwe-insulin. Izigulana ezinesifo seswekile ezifumana unyango lwe-insulin zinokukhula ukumelana ne-insulin ngenxa yokuveliswa kwezilwa-buhlungu ze-insulin, eziphawulwa ngokunyuka kwedosi ye-insulin kodwa ulawulo lweswekile yegazi lungonelisiyo. Ngeli xesha, i-insulin antibodies kufuneka ivavanywe; iziphumo ezilungileyo okanye ukwanda kwee-titers kunokusebenza njengobungqina obunenjongo bokumelana ne-insulin. Ukongezelela, oku kufunyaniswa kudlala indima encedisayo ekuxilongweni kwe-Insulin Autoimmune Syndrome (IAS).

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

Umgaqo

Le khithi ibona i-anti-insulin antibodies (IgG) kwiisampulu zeserum yabantu ngokusekelwe kwindlela engathanga ngqo, kunye ne-insulin yabantu ecocekileyo esetyenziswa njenge-antigen yokwambathisa.

Inkqubo yokuvavanya iqala ngokudibanisa isampuli ye-serum kwimithombo yokusabela efakwe ngaphambili kunye ne-antigen, ilandelwa yi-incubation. Ukuba amajoni e-insulin akhona kwisampulu, aya kuthi abophe ngokuthe ngqo kwi-insulin edityanisiweyo ye-insulin yabantu equleni, yenze ii-antigen-antibody complexes ezizinzileyo.

Emva kokuhlamba ukususa izinto ezingavumelekanga kwaye ugweme ukuphazamiseka, i-enzyme conjugates yongezwa kumaqula. Inyathelo lesibini lokufukamela livumela ezi zidibaniselwano ze-enzyme ukuba zibophe ngokuthe ngqo kwii-antigen-antibody complexes ezikhoyo. Xa isisombululo se-TMB substrate singeniswa, ukusabela kombala kwenzeka phantsi kwesenzo se-catalytic ye-enzyme kwi-complex. Ekugqibeleni, umfundi we-microplate usetyenziselwa ukulinganisa i-absorbeance (i-A value), eyenza ukuzimisela ubukho be-anti-insulin antibodies kwisampuli.

Iimpawu zeMveliso

Uvakalelo oluphezulu, ukuchaneka kunye nokuzinza

Ukucaciswa kweMveliso

Umgaqo	Uvavanyo lwe-Enzyme olunxibelelene ne-immunosorbent
Uhlobo	Ngokungathanga ngqoIndlela
Isatifikethi	NIMPA
Umzekelo	Iserum yabantu / iplasma
Inkcazo	48T /96T
Ubushushu bokugcina	2-8℃
Beka ubomi kwishelufa	12iinyanga