I-Anti-Endometrial (EM) I-Antibody ELISA Kit

I-Anti-Endometrial (EM) I-Antibody ye-ELISA Kit Umfanekiso obonakalayo

Inkcazelo emfutshane:

Le khithi isetyenziselwa ukufumanisa umgangatho we-in vitro we-anti-endometrial antibodies (EmAb) kwiserum yabantu.

I-EmAb yi-autoantibody ejolise kwi-endometrium, ivuselela iimpendulo zomzimba. Yi-antibody ephawulekayo ye-endometriosis kwaye inxulumene nokuphuphuma kwesisu kunye nokungazali. Iingxelo zibonisa i-37% -50% yezigulane ezinobudlolo, ukukhulelwa kwesisu okanye i-endometriosis yi-EmAb-positive; izinga lifikelela kwi-24% -61% kwabasetyhini emva kokuqhomfa okwenziweyo.

I-EmAb ibophelela kwii-antigens ze-endometrial, i-endometrium eyonakalisayo ngokusebenzisa i-activation ehambelanayo kunye nokugaywa kweeseli ze-immune, ukuphazamisa ukufakwa kwe-embryo kunye nokubangela ukuphuphuma kwesisu. Ihlala ihlala kunye ne-endometriosis, kunye nesantya sokubhaqa se-70% -80% kwizigulane ezinjalo. Le khithi inceda ukuxilonga i-endometriosis, ukujonga iziphumo zonyango, kunye nokuphucula iziphumo zokungachumi okuhambelanayo.

Thumela i-imeyile kuthi

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Umgaqo

Le khithi ibhaqa izilwa-buhlungu ezichasene ne-endometrial (IgG) kwiisampulu zeserum yabantu ezisekelwe kwindlela engathanga ngqo, kunye ne-antigens ye-endometrial inwebu ecocekileyo esetyenziselwa ukugquma i-microwells yangaphambili.

Inkqubo yokuvavanya iqala ngokudibanisa isampuli ye-serum kwimithombo ye-antigen-precoated reaction for incubation. Ukuba ii-anti-endometrial antibodies zikhona kwisampulu, ziya kubophelela ngokukodwa kwii-antigens ze-endometrial ezifakwe ngaphambili kwii-microwells, zenze ii-antigen-antibody complexes ezizinzileyo. Emva kokususa amacandelo angabotshwanga ngokuhlamba ukuphepha ukuphazamiseka, i-horseradish peroxidase-ebhalwe i-mouse anti-human IgG antibodies zongezwa.

Ukulandela enye incubation, ezi zithinteli zibhalwe enzyme zibophelela kwii-antigen-antibody complexes ezikhoyo. Xa i-substrate ye-TMB yongezwa, ukusabela kombala kwenzeka phantsi kwe-catalysis ye-enzyme. Ekugqibeleni, umfundi we-microplate ulinganisa ukuxhamla (ixabiso le-A), elisetyenziselwa ukumisela ubukho be-anti-endometrial antibodies (IgG) kwisampuli.

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Ukucaciswa kweMveliso

Umgaqo	Uvavanyo lwe-Enzyme olunxibelelene ne-immunosorbent
Uhlobo	Ngokungathanga ngqoIndlela
Isatifikethi	NIMPA
Umzekelo	Iserum yabantu / iplasma
Inkcazo	48T /96T
Ubushushu bokugcina	2-8℃
Beka ubomi kwishelufa	12iinyanga