I-Anti-Cyclic Citrullinated Peptide (CCP) Antibody ELISA Kit

I-Anti-Cyclic Citrullinated Peptide (CCP) i-Antibody ELISA Kit eFakelweyo

Inkcazelo emfutshane:

Le mveliso yenzelwe ukufunyanwa kwekhwalithi ye-vitro ye-anti-cyclic citrullinated antibody ye-peptide kwi-serum yabantu. Ngokweklinikhi, iyasebenza njengesixhobo esincedisayo sokuxilonga kwi-rheumatoid arthritis (RA).

Anti-cyclic citrullinated peptide antibodies are autoantibodies that target cyclic citrullinated peptides, a type of modified protein antigen. Ubukho babo buhambelana ngokusondeleyo ne-rheumatoid arthritis, isifo esingapheliyo somzimba obonakaliswe ngokuvutha kunye nomonakalo. Xa kuthelekiswa nezinye iimpawu ze-rheumatoid, ezi zithinteli-zifo zibonisa ukuchaneka okuphezulu kwe-RA, ngakumbi kumanqanaba okuqala esi sifo xa iimpawu zekliniki zingekafaneli.

Kwizigulana ezikrokrelekayo ze-RA, ukufumanisa amanqanaba e-anti-cyclic citrullinated antibody e-peptide kunokunceda ukuqinisekisa ukuxilongwa kwasekuqaleni, okubalulekileyo kungenelelo olungexesha kunye nokuthintela umonakalo odibeneyo ongenakubuyiselwa. Kwakhona kunceda ekuhlukaniseni i-RA kwezinye iintlobo ze-arthritis ezineempawu ezifanayo, ngaloo ndlela zikhokela oogqirha ukuba baphuhlise izicwangciso zonyango ezijoliswe ngakumbi kunye nokuphucula ulawulo olupheleleyo lwesifo.

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

Umgaqo

Le khithi ibona i-anti-cyclic citrullinated peptide antibodies (CCP antibodies) kwiisampulu zeserum yabantu ngokusekelwe kwindlela engathanga ngqo, kunye ne-cyclic citrullinated peptide antigens ecocekileyo esetyenziswa njenge-antigen yokwambathisa.

Inkqubo yokuvavanya iqala ngokudibanisa isampuli ye-serum kwimithombo yokusabela esele ifakwe ngaphambili kunye ne-antigens ehlanjululweyo ekhankanywe ngasentla, elandelwa lixesha lokufukamela. Ngexesha le-incubation, ukuba ii-antibodies ze-CCP zikhona kwisampuli, ziya kuqonda ngokuthe ngqo kwaye zibophe kwi-cyclic citrullinated peptide antigens ezigqunywe kwi-microwells, zenza ii-antigen-antibody complexes ezizinzileyo. Ukuqinisekisa ukuchaneka kwamanyathelo alandelayo, amacandelo angabotshwanga kwimithombo yokusabela asuswe ngenkqubo yokuhlamba, enceda ukuphelisa ukuphazamiseka okunokwenzeka kwezinye izinto kwi-serum.

Emva koko, i-enzyme conjugates yongezwa kwimithombo yokusabela. Emva kokufukanyelwa okwesibini, ezi conjugates ze-enzyme ziya kuthi ngqo kwii-antigen-antibody complexes ezikhoyo, zenze i-immune complex complex equka i-antigen, i-antibody, kunye ne-enzyme conjugate. Xa isisombululo se-TMB substrate singeniswa kwinkqubo, i-enzyme kwi-conjugate ibangela ukusabela kweekhemikhali kunye ne-substrate ye-TMB, okubangelwa utshintsho olubonakalayo lombala. Ukuqina kolu tshintsho lombala kuhambelana ngokuthe ngqo nobungakanani be-CCP antibodies ekhoyo kwisampulu ye-serum yokuqala. Ekugqibeleni, umfundi we-microplate usetyenziselwa ukulinganisa i-absorpension (i-A value) yomxube wokuphendula. Ngokuhlalutya le xabiso le-absorbection, inqanaba le-CCP antibodies kwisampulu inokumiselwa ngokuchanekileyo, inika isiseko esithembekileyo sovavanyo olufanelekileyo lwekliniki kunye nokuxilongwa.

Iimpawu zeMveliso

Uvakalelo oluphezulu, ukuchaneka kunye nokuzinza

Ukucaciswa kweMveliso

Umgaqo	Uvavanyo lwe-Enzyme olunxibelelene ne-immunosorbent
Uhlobo	Ngokungathanga ngqoIndlela
Isatifikethi	NIMPA
Umzekelo	Iserum yabantu / iplasma
Inkcazo	48T /96T
Ubushushu bokugcina	2-8℃
Beka ubomi kwishelufa	12iinyanga