Ukuxilongwa kwe-HFRS-I-Hemorrhagic Fever ene-Renal Syndrome

Imvelaphi

Intsholongwane ye-Hantaan (HV) yeyona pathogen iphambili ejongene neHemorrhagic Fever eneRenal Syndrome (HFRS). I-HFRS sisifo esosulelayo esisasazwa kwihlabathi jikelele esiphawulwa ngumkhuhlane, ukopha, kunye nokungasebenzi kakuhle kwezintso. Esi sifo siqala ngokukhawuleza, ukukhula ngokukhawuleza, kunye nezinga eliphezulu lokufa, libeka ingozi enkulu kwimpilo yoluntu. Iimpuku (ezifana ne-Apodemus agrarius kunye ne-Rattus norvegicus) zezona ndawo zigcina amanzi kunye ne-vectors ze-HV. Usulelo oluya ebantwini lwenzeka ikakhulu nge-aerosolized excreta (umchamo, ilindle, amathe), ukudibana ngqo, okanye ukulunywa yi-vector. I-HFRS ingenzeka unyaka wonke, kwaye abantu ngokubanzi banokuchaphazeleka. Ngokwezibalo ze-WHO, amazwe angama-32 kwihlabathi jikelele axele ukuqhambuka kwe-HV, ngokuxhaphaka kakhulu eMpuma Asia, eYurophu, nakwiiBalkans.

Iimpawu ze-Antibody Post Usulelo lwe-HV

Ukulandela usulelo lwe-HV, inkqubo yomzimba yokuzikhusela ivelisa izilwa-buhlungu ezithile, ngokuyintloko i-HV-IgM kunye ne-HV-IgG.

● Izilwa-buhlungu ze-HV-IgM: Zisebenza njenge-serological marker yosulelo lwakwangoko, oludla ngokubonakala kwiintsuku nje ezimbalwa emva kokuqala kweempawu, kwaye zibalulekile kuxilongo lwenqanaba elibukhali.

● Izilwa-buhlungu ze-HV-IgG: Zivela kamva kwaye zingaqhubeka ubomi bonke, zibonisa usulelo oludlulileyo okanye ukuchacha. Ukonyuka okuphindwe kane okanye ngaphezulu kwe-antibody titer ye-HV-IgG phakathi kweesampulu zeserum ebukhali kunye neyokuphola kukwaluxilongo losulelo olubukhali.

Iindlela eziqhelekileyo zokuxilongwa kwe-HV

Iindlela zangoku zaselabhoratri zokufunyanwa kwe-HV zibandakanya ukuhlukaniswa kwentsholongwane, i-PCR, i-SERological ELISA, kunye ne-colloidal gold immunoassays.

● Ukukhuliswa kwentsholongwane kunye ne-PCR zinika iinkcukacha ezithile eziphezulu kodwa zidla ixesha, zifuna ngobuchule, kwaye zifuna izibonelelo zaselebhu eziphucukileyo, zinciphisa ukusetyenziswa kwazo ngokubanzi.

● I-Micro-immunofluorescence (MIF) inika ukuchaneka okuhle kodwa ifuna imakroskopu ye-fluorescence kunye nokutolika kwengcali, inciphisa ukusetyenziswa kwesiqhelo.

● I-ELISA kunye ne-colloidal assays zegolide zamkelwa ngokubanzi kwizicwangciso zeklinikhi ngenxa yokulula kwazo, isantya, uvakalelo oluphezulu kunye neenkcukacha, kunye nokulula kokuqokelela isampuli (i-serum / plasma).

Ukusebenza kweMveliso

I-Beier Bio's HV-IgM/IgG (ELISA) Iimpawu zokuHlola

● Uhlobo lweSampuli: ISerum, iplasma

● Isampulu yoDilution: Zombini i-IgM kunye ne-IgG uvavanyo zisebenzisa isampulu yentsusa kakuhle ene-1:11 dilution (100µl isampulu yediluent + 10µl isampulu), iphucula ukuhamba komsebenzi kunye nokunciphisa umthwalo womsebenzisi.

● I-Reagent eLungele ukuSetyenziswa: Zonke ii-reagents zilungile ngaphandle kokuhlamba i-buffer (20× egxininiswe). Umbala-khowudi ukuze uchongwe lula

● Inkqubo ye-Incubation: 30 min / 30 min / 15 min; iyazenzekela ngokupheleleyo

● Ukufunyanwa kweWavelength: 450 nm kunye ne-630 nm reference

● Imichilo egqunyiweyo: Amaqula angama-96 okanye angama-48 aqhawukayo, ngalinye linekhowudi yemveliso eprintiweyo yokulandeleka kunye nokulula.

I-Beier Bio ye-HV-IgM/IgG (i-Colloidal Gold) Iimpawu zokuHlola

● Uhlobo lweSampuli: ISerum

● Ixesha lokuFumana: Iziphumo kwimizuzu eyi-15; akukho zixhobo zongezelelweyo ezifunekayo; ilungele uvavanyo olukhawulezayo kwizigulane ezingalaliswayo, ezingxamisekileyo, kunye neendawo ezisasazekileyo zezigulane

● Inkqubo: Yongeza isampuli ye-10µl kwisampulu yekhadi lovavanyo kakuhle usebenzisa idropha; Tolika iziphumo kwimizuzu eli-15 ukuya kwengama-20

Ukusebenza kweKlinikhi ye-HV-IgM (ELISA), i-HV-IgG (ELISA), kunye ne-HV-IgM/IgG (i-Colloidal Gold)